Abstract

Application of single-cell RNA sequencing (scRNA-seq) in plants improved the understanding of cell heterogeneity in various plant species, such as Arabidopsis, rice, maize, cotton, peanut and poplar.

Bread wheat (Triticumaestivum L., AABBDD, 2n = 6x = 42) is allohexaploid and evolved from the interspecific hybridizations of three distinct diploid species, Triticum urartu (AA), Aegilops speltoides (BB) and Aegilops tauschii (DD) contributing the AA, BB and DD genome, respectively.

Here, we performed single nuclei RNA sequencing (snRNA-seq) and ATAC sequencing (snATAC-seq) in wheat root to study the asymmetric gene transcription, discover cell differentiation trajectories and cell type specific gene regulatory networks.