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CRISPR design
PAM
NGG (SpCas9 from Streptococcus pyogenes: 5'-NGG-3')
TTTN (AsCpf1 from Acidaminococcus or LbCpf1 from Lachnospiraceae: 5'-TTTN-3')
max mismatch
2
0
1
3
4
5
Target Genome
Triticum aestivum (IWGSCv1.1)
Oryza sativa subsp. indica MH63
Oryza sativa Japonica (IRGSP-1.0)
Zea mays (AGPv4)
Locus Tag
% for some examples
Position
e.g. Chr3:18864113..18864812
OR
Sequence
fasta
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