CRISPR efficiency estimate

This module has two basic functions:

1.Identifying On-target Efficiency Score from custom sgRNAs;

2.Identifying sgRNAs from custom long squences and then estimate thise sgRNAs with On-target Efficiency Score.

user can input custom sgRNA multi-sequences in FASTA that more than 30 nt (included PAM "NGG"):

Custom sgRNA sequencnce in format "NNNN+spacer+NGG+NNN ", or long sequences containing one or more sgRNA sequence of the format